FBISE, NCP & NBF-aligned resources for Classes 9–12
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Biology Chapter 7 : Test Yourself
1 / 20
Which statement about enzymes is true?
Reasoning: Enzymes are biological catalysts and, according to Class 9 biology, they are proteins. However, not all proteins function as enzymes.
2 / 20
Lock and key hypothesis of enzyme action supports that:
Reasoning: The lock-and-key model states that the enzyme's active site has a fixed (rigid) shape that exactly matches the substrate, just as a key fits a specific lock.
3 / 20
Four graphs A–D show enzyme reaction rate against temperature up to 40°C. Which graph correctly rises to an optimum near 40°C and then drops sharply as the enzyme denatures?
Reasoning: As temperature increases, the rate of an enzyme-controlled reaction rises steadily due to increased kinetic energy, peaking at an optimum temperature around 40°C. Beyond this point, the reaction rate drops sharply because the high thermal energy disrupts the enzyme's structure, causing it to denature and lose its catalytic function.
4 / 20
What is true about cofactors?
Reasoning: Cofactors are non-protein substances that assist enzymes in carrying out their catalytic functions efficiently.
5 / 20
Change in pH can alter the active site by affecting the:
Reasoning: Changes in pH affect the ionization of amino acids in the enzyme, altering the shape and function of the active site.
6 / 20
The catalytic region of an enzyme recognizes and binds the substrate and carries the reaction. This region is called:
Reasoning: The active site is the specific region of an enzyme where the substrate binds and the chemical reaction takes place.
7 / 20
A graph shows enzyme X active mainly at pH 1–4, enzyme Y around pH 5–9, and enzyme Z around pH 6–10. Which statement is correct?
Reasoning: Looking at the graph, a pH value of 7 sits directly within the activity curves of both enzyme Y and enzyme Z, meaning both are functional at this level. In contrast, enzyme X is completely inactive past pH 4, and neither Y nor Z shows any activity down at pH 4.
8 / 20
In an amylase diagram, P labels the starch substrate, Q the active site, R the smaller sugar products, and S the unchanged enzyme. Which option correctly identifies P, Q, R, and S?
Reasoning: The diagram illustrates the lock-and-key model of enzyme action, where S is the unchanged enzyme (amylase) and Q is its specific active site where the reaction takes place. P represents the large substrate molecule (starch chain) entering the reaction, which is broken down into smaller product molecules labeled R (maltose units).
9 / 20
Glycolysis is the breakdown of:
Reasoning: Glycolysis is the first stage of respiration in which one molecule of glucose is broken down into pyruvic acid.
10 / 20
The mechanism of ATP synthesis is:
Reasoning: ATP is formed when a phosphate group is added to ADP. This process is called phosphorylation.
11 / 20
In aerobic respiration pyruvic acid changes to:
Reasoning: Before entering the Krebs cycle, pyruvic acid is converted into Acetyl CoA in the mitochondria.
12 / 20
Which of these uses oxygen as the final acceptor?
Reasoning: In aerobic respiration, oxygen acts as the final electron acceptor in the electron transport chain.
13 / 20
Which of these produces carbon dioxide?
Reasoning: Carbon dioxide is released during the Krebs cycle when Acetyl CoA is oxidized.
14 / 20
What are the products of light reactions in photosynthesis?
Reasoning: Light reactions produce ATP and NADPH for the Calvin cycle and release oxygen as a by-product from the splitting of water.
15 / 20
In a chloroplast overview, molecule I is water entering the light reactions, II is oxygen leaving them, III is carbon dioxide entering the Calvin cycle, and IV is sugar leaving it. Which numbered molecule is an organic biomolecule?
Reasoning: Molecule IV represents sugar / glucose, which is the primary organic biomolecule produced as the end-product of the Calvin cycle during photosynthesis. The other components are inorganic: I is water (H₂O), II is oxygen gas (O₂), and III is carbon dioxide (CO₂).
16 / 20
Which feature helps plants to make most food by the process of photosynthesis?
Reasoning: Broad and flat leaves provide a large surface area to absorb maximum sunlight, which increases the rate of photosynthesis and food production.
17 / 20
A pond weed in a water-filled test tube releases oxygen bubbles. Which conditions would produce the greatest number of bubbles?
Reasoning: The gas bubbles being produced by the pond weed are oxygen (O₂), which is a byproduct of photosynthesis. To produce more bubbles, the rate of photosynthesis needs to be maximized, which requires an abundance of raw materials like dissolved CO₂, high-energy bright light, and a warm temperature to optimize the activity of the plant's photosynthetic enzymes.
18 / 20
Which of the following processes is used by plants to make oxygen during the process of photosynthesis?
Reasoning: During the light reactions of photosynthesis, water molecules are split by light energy (photolysis), releasing oxygen, hydrogen ions, and electrons. The oxygen is released into the atmosphere.
19 / 20
A cellular-respiration overview labels X as ATP energy outputs, Y as the carrier produced in glycolysis, pyruvate oxidation, and the Krebs cycle, and Z as the carrier produced in the Krebs cycle only. Which label represents NADH?
Reasoning: The label Y represents NADH because it is produced across multiple stages of cellular respiration: 2 molecules during glycolysis, 2 during pyruvate oxidation, and 6 during the Krebs cycle, all heading into the electron transport chain. In contrast, X represents ATP energy bursts, and Z represents the 2 molecules of FADH₂ generated exclusively inside the Krebs cycle.
20 / 20
A child left a carton on the lawn for two days. When the carton was picked up, the grass under it had turned yellow. What caused the grass to change colour?
Reasoning: The carton blocked sunlight from reaching the grass. Without light, the grass could not carry out photosynthesis properly, and chlorophyll began to break down, causing the grass to turn yellow.
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